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1.

Immobilized Organo-Mercurial Lyase on Zeolite Using a Solid Binding Peptide Damien N. McCarthy, Grant C. Edwards International Journal of Environmental Bioremediation & Biodegradation. 2018 6 (1). doi: 10.12691/ijebb-6-1-2 Keywords: merB, methylmercury, enzyme immobilization, demthylation Context: ... the construct. The DNA was synthesized commercially, and expressed in E.coli (BL21DE3 Star) using pET100® vector. Sanger sequencing was used to confirm construct in transformed cells using standard T7 oligos. Expression was lactose induced, and SDS-PAGE electrop... Abstract Full Text (PDF) [Epub] Full Text (HTML)

2.

Mutational Difference between the Microsatellite BAT 26 and the TP53 Coding Gene and Survival of Patients with Oral Cavity Cancers in Senegal Mame Diarra Samb, Mbacke Sembene American Journal of Cancer Prevention. 2025 12 (1). doi: 10.12691/ajcp-12-1-2 Keywords: oral, cavity, cancer, mutations, TP53, BAT 26, survival Context: ...les from patients and 40 blood samples from healthy controls were analyzed. DNA extraction, polymerase chain reaction (PCR), and sequencing were performed to obtain genetic sequences. The software BioEdit and DnaSP were used to analyze polymorphism and genetic diversity... Abstract Full Text (PDF) [Epub] Full Text (HTML)

3.

Degradation Potential and Enzyme Characterization of Aspergillus Flavus SKF8 Isolated from a Textile Industrial Effluent against Reactive Yellow 145 Baby Jooju, Sheela Thangaraj, Senthil Kumar Sadasivam Applied Ecology and Environmental Sciences. 2022 10 (6). doi: 10.12691/aees-10-6-7 Keywords: Reactive Yellow 145, Aspergillus flavus, Biodegradation, Textile effluent, azo dyes Context: ...lem district, Tamil Nadu, India and the strain was identified as Aspergillus flavus SKF8 based on molecular methods (rDNA sequencing ). This strain demonstrated a growth dependent biodegradation activity of the Reactive Yellow 145 in which the highest rate of dye ... Abstract Full Text (PDF) [Epub] Full Text (HTML)

4.

Mundticin KS: Characterization and Production Method Alena Abaimova, Nikolay Kartsev, Edvard Svetoch, Olga Tazina, Tatiana Novikova, Mikhael Platonov, Irina Mitsevich, Maria Kanashenko, Rostislav Zhumakaev, Konstantin Detushev, Marat Teymurazov Journal of Applied & Environmental Microbiology. 2021 9 (1). doi: 10.12691/jaem-9-1-3 Keywords: bacteriocin, Mundticin KS, purification methods, antibacterial spectrum Context: ... enzymes as amylase, lipase, and papain do not have any effect on its activity. Mass spectral analysis, electrophoresis, and DNA sequencing showed the high range similarity of mundticin KS produced by strains E. mundtii B-8398 to other previously studied mundtici... Abstract Full Text (PDF) [Epub] Full Text (HTML)

5.

Metastatic Colorectal Cancer to the Right Ventricle Jamila Asif, Ahmad El-Gammal, Jad Said, Crystal Seudeal, Wehbi Hanayni, Steven Werns American Journal of Medical Case Reports. 2023 11 (2). doi: 10.12691/ajmcr-11-2-5 Keywords: colorectal cancer, cardiac metastasis, Adenocarcinoma, right ventricle/metastasis, pathology Context: ...prognosis of CRC. Given the asymptomatic presentation of cardiac metastasis in the context of CRC, future integration of genetic sequencing may help delineate further guidelines on screening for cardiac metastasis.... Abstract Full Text (PDF) [Epub] Full Text (HTML)

6.

Drugs Susceptibility Testing in Leprosy Patients from Côte d’Ivoire Reveals Multidrugs Resistance Combination Cases to Dapsone, Rifampicin and Ofloxacin Coulibaly N’Golo David, Dehe Bahou Roger, Kakou-N’Gazoa Solange, Kouakou henry, Amon Aby Christiane, Sylla Aboubacar, Bidie Alain Dit Philippe, Bamba Vagamon, Dosso Mireille American Journal of Microbiological Research. 2020 8 (4). doi: 10.12691/ajmr-8-4-6 Keywords: leprosy, M. leprae, multidrug resistance, Côte d’Ivoire Context: ...stance has been reported in several leprosy endemic region. The drug susceptibility testing was done by detecting mutation after sequencing of the drug resistance determining region. Côte d'Ivoire like many African countries has reaching the threshold of elimination of ... Abstract Full Text (PDF) [Epub] Full Text (HTML)

7.

Rapid High Specific Method for the Detection of Pseudomonas fluorescens Mohammed Abdullatif Hamod American Journal of Microbiological Research. 2015 3 (5). doi: 10.12691/ajmr-3-5-2 Keywords: Ps. fluorescens, cumene dioxygenase, PCR Context: ...mirabilis, klebsiella pneumonia and Bacillus sp) were collected and their detection were confirmed by 16S rRNA sequencing . Scanning the complete genome of Ps. fluorescens available in GenBank have denoted for the distinction of cumene dio... Abstract Full Text (PDF) [Epub] Full Text (HTML)

8.

Isolation and Cloning of sprA Gene Alkaline Protease Activity from Streptomyces flavogriseus ADEM7 and Characterization of Its Antioxidant and Antitumor Activities Doaa E. El-Hadedy, Nesreen A. Safwat, Abir Pertila, Rafat A. Siddiqui Journal of Food and Nutrition Research. 2023 11 (5). doi: 10.12691/jfnr-11-5-2 Keywords: Streptomyces flavogriseus, alkaline protease, antioxidant and anticancer, gamma radiation Context: ...olymerase chain reaction followed by cloning in PGEM-T easy vector, transformation in E. coli JM109, and direct construct sequencing . The sprA gene encoding streptogrisin a (alkaline protease-like activity) was isolated in this study and recorded in the ge... Abstract Full Text (PDF) [Epub] Full Text (HTML)

9.

Putative Amplification of 16S rRNA Markers of Escherichiacoli and Salmonellaspp Isolates From Raw Milk Sample of Cattle Adeoti O.M, Fehintola O.I, Adeoye K.A, Olaoye O.J., Olufemi S.O, Adedokun E.O, Adeshina D.A American Journal of Biomedical Research. 2020 8 (1). doi: 10.12691/ajbr-8-1-1 Keywords: 16S rRNA, diary, susceptibility-test, virulence, amplicons, marker genes Context: ... using the disk diffusion method. A more specific identification of the bacterial 16S rRNA genes was performed by PCR and Sanger sequencing technique by using specific primers which are specific for the amplification of E.coli and Salmonella genes. Total viable bacteria... Abstract Full Text (PDF) [Epub] Full Text (HTML)

10.

Characterization of Psychrophilic Pathogens in Ready-to-eat Salads Sold in Supermarkets in the City of Abidjan (Côte d’Ivoire) N’Goran Parfait N’Zi, Hadja Djénéba Ouattara, Yao Paul Attien, Valérie Carole Gbonon, Nathalie Kouadio Guessennd, Djédoux Maxime Angaman Journal of Food Security. 2022 10 (3). doi: 10.12691/jfs-10-3-1 Keywords: ready-to-eat salads, psychrophilic pathogens, Abidjan (Côte d’Ivoire) Context: ...evealed the presence of similar germs in 6 samples based on growth characters with Oxford’s selective medium. However, molecular sequencing allowed to identify six (6) different pathogens, Lysinibacillus fusiformis in spinach salads (EP), Listeria monocytogene... Abstract Full Text (PDF) [Epub] Full Text (HTML)