Figure 3. PU reactivates impaired autophagy: (A) Western blotting analyzed the conversion of autophagy LC3-I to LC3-II in vitro. (B) PU promoted the degradation of p62. Cells were treated with PA and/or PU in the presence or absence of CHX and western blotting was used to analyze the changes in p62. (C) In the presence of PA, PU increases autophagy flux. The cells were treated with the corresponding reagent for 24 h in the presence or absence of bafilomycin A1, and then analyzed for LC3 by western blotting

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Punicalagin Inhibits Palmitate Acid-induced Lipoapoptosis through Inhibition of ER Stress, and Activation of SIRT1/Autophagy in HepG2 Cells

Han Zhang, Hao Wu, Shutao Yin, Hongbo Hu, Chong Zhao

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