Figure 3. The MCR extract, paeoniflorin, and oxypaeoniflorin suppress the production of inflammatory cytokines and their mRNA expression in LPS-induced RAW264.7 cells. Cells were pre-treated with the MCR extract, paeoniflorin, or oxypaeoniflorin for 4 h and then stimulated for 18 h with LPS (1 μg/ml). The production of inflammatory cytokines in supernatants was measured using ELISA kit at 450 nm and 560 nm. (A) The production of IL-1β; (B) The production of IL-6; (C) The production of TNF-α. (D) The mRNA expression of pro-inflammatory cytokines was measured by RT-PCR. Values with different letters are significantly different, p < 0.05. The experiment was performed in tetraplicate and triplicate : Anti-inflammatory Effects of Moutan Cortex Radicis Extract, Paeoniflorin and Oxypaeoniflorin through TLR Signaling Pathway in RAW264.7 Cells : Science and Education Publishing

Figure 3. The MCR extract, paeoniflorin, and oxypaeoniflorin suppress the production of inflammatory cytokines and their mRNA expression in LPS-induced RAW264.7 cells. Cells were pre-treated with the MCR extract, paeoniflorin, or oxypaeoniflorin for 4 h and then stimulated for 18 h with LPS (1 μg/ml). The production of inflammatory cytokines in supernatants was measured using ELISA kit at 450 nm and 560 nm. (A) The production of IL-1β; (B) The production of IL-6; (C) The production of TNF-α. (D) The mRNA expression of pro-inflammatory cytokines was measured by RT-PCR. Values with different letters are significantly different, p < 0.05. The experiment was performed in tetraplicate and triplicate

Journal of Food and Nutrition Research. 2018, 6(1), 26-31 doi:10.12691/jfnr-6-1-5