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Fig
ure
3.
The MCR extract, paeoniflorin, and oxypaeoniflorin suppress the production of inflammatory cytokines and their mRNA expression in LPS-induced RAW264.7 cells. Cells were pre-treated with the MCR extract, paeoniflorin, or oxypaeoniflorin for 4 h and then stimulated for 18 h with LPS (1 μg/ml). The production of inflammatory cytokines in supernatants was measured using ELISA kit at 450 nm and 560 nm. (A) The production of IL-1β; (B) The production of IL-6; (C) The production of TNF-α. (D) The mRNA expression of pro-inflammatory cytokines was measured by RT-PCR. Values with different letters are significantly different,
p
< 0.05. The experiment was performed in tetraplicate and triplicate
From
Anti-inflammatory Effects of Moutan Cortex Radicis Extract, Paeoniflorin and Oxypaeoniflorin through TLR Signaling Pathway in RAW264.7 Cells
Chang-Kil Yoo, Ji-Hyun Hwang, Kippeum Lee, Young-Jin Lee, Kui-Jin Kim, Boo-Yong Lee
Journal of Food and Nutrition Research
.
2018
, 6(1), 26-31 doi:10.12691/jfnr-6-1-5
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