Figure 4. The MCR extract, paeoniflorin, and oxypaeoniflorin attenuate the downstream targets of TLR4 signaling pathway in LPS-induced RAW264.7 cells. Cells were pre-treated with the MCR extract, paeoniflorin, or oxypaeoniflorin for 4 h and then stimulated with LPS (1 μg/ml). The mRNA expression of pro-inflammatory cytokines was measured by RT-PCR. The protein level of MAPKs was measured using western blot. The experiment was performed in triplicate


Anti-inflammatory Effects of Moutan Cortex Radicis Extract, Paeoniflorin and Oxypaeoniflorin through TLR Signaling Pathway in RAW264.7 Cells

Chang-Kil Yoo, Ji-Hyun Hwang, Kippeum Lee, Young-Jin Lee, Kui-Jin Kim, Boo-Yong Lee

Journal of Food and Nutrition Research. 2018, 6(1), 26-31 doi:10.12691/jfnr-6-1-5