Figure 2. Effect of the MCR extract, paeoniflorin, and oxypaeoniflorin on nitric oxide (NO) production and protein expression of iNOS in LPS-induced RAW264.7 cells. Cells were pre-treated with the MCR extract (50 and 100 μg/ml), paeoniflorin (10 and 30 μM), and oxypaeoniflorin (10 and 30 μM) for 4 h and then co-treated with LPS (1 μg/ml) for 18 h. (A) the production of NO in LPS-induced RAW264.7 cells with the presence or absence of the MCR extract, paeoniflorin and oxypaeoniflorin using the Griess reagent at 540 nm. (B) The expression of iNOS mRNA using RT-PCR. (C) The expression of iNOS protein using western blot. Values with different letters are significantly different, p < 0.05. The experiment was performed in triplicate : Anti-inflammatory Effects of Moutan Cortex Radicis Extract, Paeoniflorin and Oxypaeoniflorin through TLR Signaling Pathway in RAW264.7 Cells : Science and Education Publishing

Figure 2. Effect of the MCR extract, paeoniflorin, and oxypaeoniflorin on nitric oxide (NO) production and protein expression of iNOS in LPS-induced RAW264.7 cells. Cells were pre-treated with the MCR extract (50 and 100 μg/ml), paeoniflorin (10 and 30 μM), and oxypaeoniflorin (10 and 30 μM) for 4 h and then co-treated with LPS (1 μg/ml) for 18 h. (A) the production of NO in LPS-induced RAW264.7 cells with the presence or absence of the MCR extract, paeoniflorin and oxypaeoniflorin using the Griess reagent at 540 nm. (B) The expression of iNOS mRNA using RT-PCR. (C) The expression of iNOS protein using western blot. Values with different letters are significantly different, p < 0.05. The experiment was performed in triplicate

Journal of Food and Nutrition Research. 2018, 6(1), 26-31 doi:10.12691/jfnr-6-1-5