Figure 2. Effect of DHA on osteoclast formation in mouse bone marrow cell culture. Bone marrow cells were pre-cultured with M-CSF (30 ng/ml) for 3 days and then cultured with M-CSF (30 ng/ml) and RANKL (30 ng/ml) in the presence or absence of different concentrations of DHA for 6 days. (A) TRAP(+)MNCs for DHA treatment; (B) TRAP activity for DHA treatment. Data are expressed as mean ± SEM of 3 independent experiments. a,b,c,dMeans sharing the same letters are not significantly different, p < 0.05

From

Combined Effects of Soy Isoflavones and Docosahexaenoic Acid on Osteoclast Formation

Shinichi Katsumata, Gabrielle G. Plimmer, Miki Tadaishi, Yuko Tousen, Yoshiko Ishimi, Marlena C. Kruger

Journal of Food and Nutrition Research. 2016, 4(7), 429-435 doi:10.12691/jfnr-4-7-3