Figure 3. Combined effect of soy isoflavones and DHA on osteoclast formation in mouse bone marrow cell culture. Bone marrow cells were pre-cultured with M-CSF (30 ng/ml) for 3 days and then cultured with M-CSF (30 ng/ml) and RANKL (30 ng/ml) in the presence or absence of 10 μM daidzein or genistein with or without 20 μM DHA for 6 days. (A) TRAP(+)MNCs for combination of isoflavones and DHA treatment; (B) TRAP activity for combination of isoflavones and DHA treatment. Data are expressed as mean ± SEM of 3 independent experiments. Means sharing the same letters are not significantly different, p < 0.05 (a,b,c,dSmall letters: Control vs Daidzein, A,B,C,DCapital letters: Control vs Genistein)

From

Combined Effects of Soy Isoflavones and Docosahexaenoic Acid on Osteoclast Formation

Shinichi Katsumata, Gabrielle G. Plimmer, Miki Tadaishi, Yuko Tousen, Yoshiko Ishimi, Marlena C. Kruger

Journal of Food and Nutrition Research. 2016, 4(7), 429-435 doi:10.12691/jfnr-4-7-3