Figures index

From

Combined Effects of Soy Isoflavones and Docosahexaenoic Acid on Osteoclast Formation

Shinichi Katsumata, Gabrielle G. Plimmer, Miki Tadaishi, Yuko Tousen, Yoshiko Ishimi, Marlena C. Kruger

Journal of Food and Nutrition Research. 2016, 4(7), 429-435 doi:10.12691/jfnr-4-7-3
  • Figure 1. Effect of soy isoflavones on osteoclast formation in mouse bone marrow cell culture. Bone marrow cells were pre-cultured with M-CSF (30 ng/ml) for 3 days and then cultured with M-CSF (30 ng/ml) and RANKL (30 ng/ml) in the presence or absence of different concentrations of daidzein or genistein for 6 days. (A) TRAP(+)MNCs for daidzein treatment; (B) TRAP(+)MNCs for genistein treatment; (C) TRAP activity for daidzein treatment; (D) TRAP activity for genistein treatment. Data are expressed as mean ± SEM of 3 independent experiments. a,b,cMeans sharing the same letters are not significantly different, p < 0.05
  • Figure 2. Effect of DHA on osteoclast formation in mouse bone marrow cell culture. Bone marrow cells were pre-cultured with M-CSF (30 ng/ml) for 3 days and then cultured with M-CSF (30 ng/ml) and RANKL (30 ng/ml) in the presence or absence of different concentrations of DHA for 6 days. (A) TRAP(+)MNCs for DHA treatment; (B) TRAP activity for DHA treatment. Data are expressed as mean ± SEM of 3 independent experiments. a,b,c,dMeans sharing the same letters are not significantly different, p < 0.05
  • Figure 3. Combined effect of soy isoflavones and DHA on osteoclast formation in mouse bone marrow cell culture. Bone marrow cells were pre-cultured with M-CSF (30 ng/ml) for 3 days and then cultured with M-CSF (30 ng/ml) and RANKL (30 ng/ml) in the presence or absence of 10 μM daidzein or genistein with or without 20 μM DHA for 6 days. (A) TRAP(+)MNCs for combination of isoflavones and DHA treatment; (B) TRAP activity for combination of isoflavones and DHA treatment. Data are expressed as mean ± SEM of 3 independent experiments. Means sharing the same letters are not significantly different, p < 0.05 (a,b,c,dSmall letters: Control vs Daidzein, A,B,C,DCapital letters: Control vs Genistein)
  • Figure 4. Combined effect of soy isoflavones and DHA on NFATc1 and TRAP mRNA expression in mouse bone marrow cell culture. Bone marrow cells were pre-cultured with M-CSF (30 ng/ml) for 3 days and then cultured with M-CSF (30 ng/ml) and RANKL (30 ng/ml) in the presence or absence of 10 μM daidzein or genistein with or without 20 μM DHA for 6 days. (A) NFATc1; (B) TRAP. Data are expressed as mean ± SEM of 3 independent experiments. Means sharing the same letters are not significantly different, p < 0.05 (a,bSmall letters: Control vs Daidzein, A,B,CCapital letters: Control vs Genistein)
  • Figure 5. Combined effect of soy isoflavones and DHA on osteoclast formation in RAW 264.7 cell culture. RAW 264.7 cells were cultured with RANKL (15 ng/ml) in the presence or absence of 10 μM daidzein or genistein with or without 20 μM DHA for 5 days. Data are expressed as mean ± SEM of 3 independent experiments. Means sharing the same letters are not significantly different, p < 0.05 (a,bSmall letters: Control vs Daidzein, A,B,CCapital letters: Control vs Genistein)