Figure 2. (A) Reversed-phase HPLC pattern on a Primesphere 10 C₁₈ column of active fraction from Fr IV and HPLC operation was carried out with 15% acetonitrile as mobile phase at 1 mL/min of a flow rate using UV detector at 215 nm. (B). Inhibition NO production activities of fractions of Reversed-phase HPLC pattern on a Primesphere 10 C18 column chromatography. Error bars represent mean and S.D from triplicate experiments after deduction of background values from raw values. (C) Further separation of active fraction peak responsible for the highest production activity was finally purified on a Synchropak RPP-100 analytical column. HPLC operation was carried out with 10% acetonitrile as mobile phase at 1 mL/min of a flow rate using UV detector at 215 nm. (D). Identification of molecular mass and amino acid sequence of AAIP. MS/MS experiments were performed on a Q-TOF tandem mass spectrometer (Micromass Co., Manchester, UK) equipped with a nano-ESI source. Sequencing of active peptide was acquired over the m/z range 50-2500 and sequenced by using the PepSeq de nove sequencing algorithm (Qian et al. (2016))

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Inhibitory Effects and Molecular Mechanism of an Anti-inflammatory Peptide Isolated from Intestine of Abalone, Haliotis Discus Hannai on LPS-Induced Cytokine Production via the p-p38/p-JNK Pathways in RAW264.7 Macrophages

Zhong-Ji Qian, BoMi Ryu, Won Sun Park, IL-Whan Choi, Won-Kyo Jung

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