Figure 3. (A). The cytocompatible effects of the AAIP on viability of RAW 264.7 cells. Cells were treated with AAIP at the indicated concentrations (0-500 μM). After 24 h to treat AAIP, cell viability was assessed by MTT assay as described in the text. Results are means ± standard error of three independent experiments. (B). Effects of AAIP on LPS-induced NO production in RAW264.7 cells. Cells cultured in serum-free media were pretreated with different concentrations (10-500 μM) of AAIP for 1 h, and then stimulated with LPS (100 ng/mL). Conditioned medium was collected after 48 h and NO concentrations were measured using the Griess reaction (Qian et al. (2016)) : Inhibitory Effects and Molecular Mechanism of an Anti-inflammatory Peptide Isolated from Intestine of Abalone, Haliotis Discus Hannai on LPS-Induced Cytokine Production via the p-p38/p-JNK Pathways in RAW264.7 Macrophages : Science and Education Publishing

Figure 3. (A). The cytocompatible effects of the AAIP on viability of RAW 264.7 cells. Cells were treated with AAIP at the indicated concentrations (0-500 μM). After 24 h to treat AAIP, cell viability was assessed by MTT assay as described in the text. Results are means ± standard error of three independent experiments. (B). Effects of AAIP on LPS-induced NO production in RAW264.7 cells. Cells cultured in serum-free media were pretreated with different concentrations (10-500 μM) of AAIP for 1 h, and then stimulated with LPS (100 ng/mL). Conditioned medium was collected after 48 h and NO concentrations were measured using the Griess reaction (Qian et al. (2016))

Journal of Food and Nutrition Research. 2016, 4(10), 690-698 doi:10.12691/jfnr-4-10-9