Figure 2. The knockdown of total TNC and TNC-AD1 in MDA-MB-231 using siRNA. A + D) MDA-MB-231 transfected with scrambled siRNA. B+ E) MDA-MB-231 transfected with total TNC siRNA. C+ F) MDA-MB-231 transfected with TNC-14-AD1 siRNA IHC staining using BC-24 monoclonal anti-TNC antibody is shown in A, B and C; whereas IHC staining using the polyclonal anti-TNC-AD1 antibody is shown in D, E and F.

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Generation and Validation of Antibodies Targeting AD1 Containing Isoform of Alternatively Spliced TNC

Ali S Alhareth, Musaad A Alsulaiman, Abo baker I Alshomrani, Waleed A Alyami, Hamad M Harthi

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