Figure 1. ICC staining for TNC-AD1 expression in cell lines. MDA-MB-231(A&D) and SK-Mel-28 (C&F) cells endogenously express TNC-AD1, whereas MCF-7 cells are TNC null (B&E). The purified antibodies generated from two immunised animals (GB1 (A,B and C); GB2 (D,E and F)) were tested for the expression of TNC-AD1. GB1 showed a clear detection of TNC-AD1, whereas GB2 did not show any expression of TNC-AD1.

From

Generation and Validation of Antibodies Targeting AD1 Containing Isoform of Alternatively Spliced TNC

Ali S Alhareth, Musaad A Alsulaiman, Abo baker I Alshomrani, Waleed A Alyami, Hamad M Harthi

American Journal of Medical and Biological Research. 2019, 7(1), 24-28 doi:10.12691/ajmbr-7-1-5