Figure 3. Western blot analysis of TNC expression using H-300 anti-TNC and two anti-AD1 antibodies. A) Expression of exogenous TNC isoforms transfected in MCF-7. B) Expression of endogenousTNC using MDA-MB-231 cell line compared to MCF-7 TNC null cell lines. Anti-vinculin antibody was used as loading control.

From

Generation and Validation of Antibodies Targeting AD1 Containing Isoform of Alternatively Spliced TNC

Ali S Alhareth, Musaad A Alsulaiman, Abo baker I Alshomrani, Waleed A Alyami, Hamad M Harthi

American Journal of Medical and Biological Research. 2019, 7(1), 24-28 doi:10.12691/ajmbr-7-1-5