Figure 5. TBE regulated the IRS-1 serine/tyrosine phosphorylation in mice skeletal muscle tissue under insulin-resistant conditions. Mice were treated by gavage with TBE at concentrations of 100, 200 and 500 mg/kg or metformin (200 mg/kg). After 30 min, mice were intraperitoneally injected with Mac-CM (0.1 mL/10g, diluted with saline, 1:1, v/v). (a) For another 30 min, muscle tissue was separated and homogenized for analyzing Serine (S307) phosphorylation of IRS-1 by Western blot. (b) For another 30 min, mice were injected with insulin (0.5 IU/kg, s.c.), and tyrosine (Py99) phosphorylation of IRS-1 was analyzed by Western blot after 30 min. Metformin was taken as a positive control. The results were expressed as the mean ± SD (n = 3). *p < 0.05, compare to control : Tartary Buckwheat Extracts Regulate Insulin Sensitivity through IKKβ/IR/IRS-1/Akt Pathway under Inflammation Condition in Mice : Science and Education Publishing

Figure 5. TBE regulated the IRS-1 serine/tyrosine phosphorylation in mice skeletal muscle tissue under insulin-resistant conditions. Mice were treated by gavage with TBE at concentrations of 100, 200 and 500 mg/kg or metformin (200 mg/kg). After 30 min, mice were intraperitoneally injected with Mac-CM (0.1 mL/10g, diluted with saline, 1:1, v/v). (a) For another 30 min, muscle tissue was separated and homogenized for analyzing Serine (S307) phosphorylation of IRS-1 by Western blot. (b) For another 30 min, mice were injected with insulin (0.5 IU/kg, s.c.), and tyrosine (Py99) phosphorylation of IRS-1 was analyzed by Western blot after 30 min. Metformin was taken as a positive control. The results were expressed as the mean ± SD (n = 3). *p < 0.05, compare to control

Journal of Food and Nutrition Research. 2014, 2(6), 321-329 doi:10.12691/jfnr-2-6-9