Figure 3. Expression genes and proteins associated with Alzheimer disease tested in HNDF and AD fibroblasts cultured in inflammatory conditions induced with TNFα. (A) Effects of test combinations of natural compounds and select pharmaceutical inhibitors on APOE, Tau, CSF, and PTGS2 gene expression at mRNA level. Expression of tested genes was performed in cells co-treated with 67 pg/ml of TNFα and either with 1.0 μg/ml of natural compounds combinations or 50 μM p38 inhibitor, 40 nM JNKs inhibitor, 100 nM ICI inhibitor, respectively, for 48h and evaluated with 0.7 μg of mRNA by RT-qPCR as described in Materials and Methods. (B) Effects of test combinations of natural compounds on APOE, Tau, and αER proteins. Protein synthesis assessed by Western blot in cells co-treated with 50 pg/ml of TNFα and either with 1.0 μg/ml of natural compounds combinations or 50 μM p38 inhibitor, 40 nM JNKs inhibitor, 100 nM ICI inhibitor, respectively, for 48h as described in Material and Methods. Statistically significant differences between treatment and control are presented as # p ≤ 0.05, ∆ p ≤ 0.01, * p ≤ 0.001; control - 0.01% DMSO. : Combinations of Selective Estrogen Receptor Modulators and Phospholipid Precursors Differentially Modulate Expression of Inflammatory Genes in Alzheimer’s Disease : Science and Education Publishing

Figure 3. Expression genes and proteins associated with Alzheimer disease tested in HNDF and AD fibroblasts cultured in inflammatory conditions induced with TNFα. (A) Effects of test combinations of natural compounds and select pharmaceutical inhibitors on APOE, Tau, CSF, and PTGS2 gene expression at mRNA level. Expression of tested genes was performed in cells co-treated with 67 pg/ml of TNFα and either with 1.0 μg/ml of natural compounds combinations or 50 μM p38 inhibitor, 40 nM JNKs inhibitor, 100 nM ICI inhibitor, respectively, for 48h and evaluated with 0.7 μg of mRNA by RT-qPCR as described in Materials and Methods. (B) Effects of test combinations of natural compounds on APOE, Tau, and αER proteins. Protein synthesis assessed by Western blot in cells co-treated with 50 pg/ml of TNFα and either with 1.0 μg/ml of natural compounds combinations or 50 μM p38 inhibitor, 40 nM JNKs inhibitor, 100 nM ICI inhibitor, respectively, for 48h as described in Material and Methods. Statistically significant differences between treatment and control are presented as # p ≤ 0.05, ∆ p ≤ 0.01, * p ≤ 0.001; control - 0.01% DMSO.

American Journal of Food and Nutrition. 2025, 13(4), 126-137 doi:10.12691/ajfn-13-4-2