Figure 1. Expression of genes and proteins associated with Alzheimer disease tested in HNDF and AD fibroblasts cultured in non-inflammatory conditions. (A) Effects of test combinations of natural compounds on APOE, Tau, CSF, and PTGS2 gene expression at mRNA level. Expression of tested genes was performed in cells treated with 1.0 μg/ml of natural compounds combinations for 48h and evaluated with 0.7 μg of mRNA by RT-qPCR as described in Material and Methods (B) Effects of test combinations of natural compounds on APOE, Tau, and αER proteins. Protein synthesis was evaluated by Western blot in cells treated with 1.0 μg/ml of natural compounds combinations for 48h as described in Materials and Methods. Statistically significant differences between treatments and control are presented as # p ≤ 0.05, ∆ p ≤ 0.01, * p ≤ 0.001; control - 0.01% DMSO. : Combinations of Selective Estrogen Receptor Modulators and Phospholipid Precursors Differentially Modulate Expression of Inflammatory Genes in Alzheimer’s Disease : Science and Education Publishing

Figure 1. Expression of genes and proteins associated with Alzheimer disease tested in HNDF and AD fibroblasts cultured in non-inflammatory conditions. (A) Effects of test combinations of natural compounds on APOE, Tau, CSF, and PTGS2 gene expression at mRNA level. Expression of tested genes was performed in cells treated with 1.0 μg/ml of natural compounds combinations for 48h and evaluated with 0.7 μg of mRNA by RT-qPCR as described in Material and Methods (B) Effects of test combinations of natural compounds on APOE, Tau, and αER proteins. Protein synthesis was evaluated by Western blot in cells treated with 1.0 μg/ml of natural compounds combinations for 48h as described in Materials and Methods. Statistically significant differences between treatments and control are presented as # p ≤ 0.05, ∆ p ≤ 0.01, * p ≤ 0.001; control - 0.01% DMSO.

American Journal of Food and Nutrition. 2025, 13(4), 126-137 doi:10.12691/ajfn-13-4-2