Figure 4. Effects of Bangle extract (Ba) on macrophage infiltration in the gastrocnemius muscle (GM) and epididymal fat tissues of high-fat diet (HFD) fed SAMP8 mice. Representative images of immunohistochemical staining for mouse macrophage-specific F4/80 in the GMs (A, B, C, and D) and epididymal fat tissues (E, F, G, and H), respectively. (A, E); Rcon (SAMR1 control), (B, F); Con (SAMP8 control), (C, G); HFD + 0% Ba, and (D, H); HFD + 2% Ba groups. Number of F4/80-positive macrophages in (I) GMs and (J) epididymal fat tissues. Arrows indicate F4/80-positive macrophages. Counterstaining with hematoxylin (scale bar: 50 μm for all images). Values are presented as the mean ± SEM (n = 6–8). aP < 0.05 compared with Rcon group. bP < 0.05 compared with Con group. cP < 0.05 compared with HFD + 0% Ba : Bangle (Zingiber purpureum) Extract Attenuates Insulin Resistance and Inflammation in the Skeletal Muscle of High Fat Diet-fed Young SAMP8 Mice : Science and Education Publishing

Figure 4. Effects of Bangle extract (Ba) on macrophage infiltration in the gastrocnemius muscle (GM) and epididymal fat tissues of high-fat diet (HFD) fed SAMP8 mice. Representative images of immunohistochemical staining for mouse macrophage-specific F4/80 in the GMs (A, B, C, and D) and epididymal fat tissues (E, F, G, and H), respectively. (A, E); Rcon (SAMR1 control), (B, F); Con (SAMP8 control), (C, G); HFD + 0% Ba, and (D, H); HFD + 2% Ba groups. Number of F4/80-positive macrophages in (I) GMs and (J) epididymal fat tissues. Arrows indicate F4/80-positive macrophages. Counterstaining with hematoxylin (scale bar: 50 μm for all images). Values are presented as the mean ± SEM (n = 6–8). ^aP < 0.05 compared with Rcon group. ^bP < 0.05 compared with Con group. ^cP < 0.05 compared with HFD + 0% Ba

Journal of Food and Nutrition Research. 2021, 9(8), 434-441 doi:10.12691/jfnr-9-8-6