Figure 6. S-nitrosylated IKK-β Expression in LPS-induced Raw264.7 cells by treated with curcumin. S-nitrosylated IKKβ as obtained from cell lysates of each experimental group at12 (A) and 24h (B) by biotin switch method and detected by Western blot using anti-biotin. The expression level of IKKβ was as the control and relative fold change of S-nitrosylated IKKβ was normalized by IKKβ protein expression. The data was repeated 3 times independently to ensure reproducibility and the standard deviation of the mean are represented as error bars (n=3). Values with different letters were significantly different (p< 0.05) at corresponding concentrations between different treatments

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S-nitrosylation of Inhibitor-κB kinase: Identifying Novel Targets of Curcumin

Ning-Jo Kao, Chien-Sheng Wu, Jia-Yuan Hu, Zwe-Ling Kong

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