Figure 7. IκBphosphorylation and NF-κB activation in LPS-induced Raw264.7 cells by treated with curcumin. The expression level of phosphorylated IκB (pIκB), IκB, and cytosolic/nuclear NF-κB proteins were detected by Western blot. Act in expression was as the loading control. The bar chart presented the relative quantification of p-IκB expression normalized by IκB protein expression The data was repeated 3 times independently to ensure reproducibility and the standard deviation of the mean are represented as error bars (n=3). Values with different letters were significantly different (p< 0.05) at corresponding concentrations between different treatments

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S-nitrosylation of Inhibitor-κB kinase: Identifying Novel Targets of Curcumin

Ning-Jo Kao, Chien-Sheng Wu, Jia-Yuan Hu, Zwe-Ling Kong

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