Figure S2. DHMBA did not alter mitochondrial biogenesis in oleate-treated C2C12 myotubes. Myotubes were treated with DHMBA (500 μM) or ES (8V/cm, 1 ms, 1 Hz) for 24 h and 400 μM oleate was also included. Cells were harvested and fixed with the 4% formaldehyde. The fixed cells were washed with PBS for two times and then stained with 20 nM MitoGreen (PromoCell GmbH, Heidelberg, Germany) at RT for 30 min. After staining, the cells were analyzed directly by FACS. Data are presented as the means ± SD of four separate experiments. N.S.: not significant by Student t-test : Improvement of Mitochondrial Function and Lipid Utilization by 3,5-dihydroxy-4-methoxybenzyl Alcohol, an Oyster-derived polyphenol, in Oleate-loaded C2C12 Myotubes : Science and Education Publishing

Figure S2. DHMBA did not alter mitochondrial biogenesis in oleate-treated C2C12 myotubes. Myotubes were treated with DHMBA (500 μM) or ES (8V/cm, 1 ms, 1 Hz) for 24 h and 400 μM oleate was also included. Cells were harvested and fixed with the 4% formaldehyde. The fixed cells were washed with PBS for two times and then stained with 20 nM MitoGreen (PromoCell GmbH, Heidelberg, Germany) at RT for 30 min. After staining, the cells were analyzed directly by FACS. Data are presented as the means ± SD of four separate experiments. N.S.: not significant by Student t-test

Journal of Food and Nutrition Research. 2016, 4(8), 498-507 doi:10.12691/jfnr-4-8-3