Figure S1. Myotube differentiation was confirmed by morphology and expression of myotube-specific Myosin heavy chain II. (A) After a 7-day differentiation, cell morphology was examed with an optical microscope. (B) Myotubes were fixed in 4% formaldehyde and permeabilized with 0.5% saponin. Mouse anti-myosin II antibody (cat. #: 14-6503, eBioscience) and PE-conjugated anti-mouse antibody (cat#: 12-4010, eBioscience) were used according to recommended procedure. Finally, the cells were incubated with PBS containing DAPI and the fluorescence was examined with the BIOREVO BZ-9000 fluorescence microscopy. The scale bar represented as 50 μm in length


Improvement of Mitochondrial Function and Lipid Utilization by 3,5-dihydroxy-4-methoxybenzyl Alcohol, an Oyster-derived polyphenol, in Oleate-loaded C2C12 Myotubes

Yi-Shing Ma, Shigeru Yoshida, Yu Kobayashi, Noriaki Kawanishi, Takayuki Furukawa, Hirotoshi Fuda, Shu-Ping Hui, Hitoshi Chiba

Journal of Food and Nutrition Research. 2016, 4(8), 498-507 doi:10.12691/jfnr-4-8-3