Figure 4. Effect of DCM fraction on the expression level of AD related NF-κB signaling proteins in HaCaT cells. HaCaT cells untreated (a), HaCaT cells induced by IFN-γ and TNF-α (each 10 ng/mL) without pretreatment with DCM fraction (b), HaCaT cells pretreated with DCM fraction and induced by IFN-γ and TNF-α (each 10 ng/mL) (c). HaCaT cells pretreated with vehicle, control (DMSO), or 80 μg/mL of DCM fraction for 2 h and then treated with 10 ng/mL of IFN-γ and TNF-α for 18 h. Relative abundances of proteins were calculated for NF-κB1, NF-κB2, RelA/p65, RelA/p65 (pS529), and c-Rel. V, vehicle (serum free medium), C, control (DMSO+IFN-γ and TNF-α)

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Orostachys japonicus Suppresses IFN-γ and TNF-α Induced Proinflammatory Chemokines in Atopic Dermatitis Relating HaCaT Cells via Down-regulation of Expression and Translocation of NF-κB

Yoo Jung Jeon, Sung Hyun Kim, Dong Seok Lee

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