Figure 3. The production levels of (A) MDC, (B) RANTES, (C) IL-8, and (D) TARC were measured using the culture supernatant of IFN-γ and TNF-α stimulated HaCaT cells. The cells were pretreated with various concentrations of DCM fraction (0, 0.1% DMSO, 10, 20, 40, and 80 μg/mL) for 2 h and then induced with IFN-γ and TNF-α (each 10 ng/mL) for 18 h. V, vehicle (serum free medium), C, control (DMSO+IFN-γ/TNF-α). The data are presented as the means ± SEM of three experiments. ∗p < 0.06 versus control

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Orostachys japonicus Suppresses IFN-γ and TNF-α Induced Proinflammatory Chemokines in Atopic Dermatitis Relating HaCaT Cells via Down-regulation of Expression and Translocation of NF-κB

Yoo Jung Jeon, Sung Hyun Kim, Dong Seok Lee

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