Figure 5. SDS-PAGE gel indicating SBP does not bind to Siliaflash or Sigma-Aldrich 60Å silica gel, or CBV100 zeolite. Lane 1. Ladder (Bio-rad broad range), Lane2. Crude extract, Lane 3. Ni purified, Lane 4. Unbound on Sigma-Aldrich gel, Lane 5. Combined wash fractions for previous, Lane 6. Eluted fraction, Lane 7. Unbound on CBV100 zeolite, Lane 8. Combined wash fractions for previous, Lane 9. Eluted fraction, Lane 10. Siliaflash gel unbound, Lane 11. Combined wash fractions for previous, Lane 12, Eluted fraction

From

Immobilized Organo-Mercurial Lyase on Zeolite Using a Solid Binding Peptide

Damien N. McCarthy, Grant C. Edwards

International Journal of Environmental Bioremediation & Biodegradation. 2018, 6(1), 8-17 doi:10.12691/ijebb-6-1-2