In this paper, we described detection of Aeromonas.hydrophila in water of AL-Jadryia river (Baghdad city, Iraq), during the period from July,2012 to April, 2013. The samples collected from water of Jaderia river and transfer to Bacteriology laboratory for diagnosis. A.hydrophila isolates which were diagnosed by three methods (Culture method, biochemical tests, Api20E system), in Culture method was isolated (71) isolate on blood agar and TCBS media and (44) isolates by biochemical tests while Api20E kit was the important method for diagnosis, which has led to isolate and diagnosis of (36) isolate of A.hydrophila. Also which used the PCR method of gene Tetracycline gene (tetA-E) was the best methods for diagnosis, which has led to isolate and diagnosis as (27) isolates of A.hydrophila have tetA-E sequence gene of all samples. The virulence factors of bacteria were detected and showed that all isolates produced of heamolysin, protease, lipase enzymes and also detection of tetracycline resistant gene of these samples.
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