Morphological, Physiological and Molecular Changes in Solanum tuberosum L. in Response to Pre-sowing Tuber Irradiation by Gamma Rays
1National Products Research Department, National Center for Radiation Research and Technology (NCRRT), Nasr City, Egypt
Tuber of two varieties of potato named Silana and Daimont were irradiated with control, 10 and 30 Gy of gamma rays before planting to investigate its effects on morphological, physiological criteria and DNA profiles. The effects of γ-irradiation on stored tubers were also investigated. It was observed that irradiation treatments improved vegetative characters in Silana and most of the physical properties were increased in response to treatment with 10Gy. But, in Daimont variety, the physical properties were inconsistently affected. Total phenols showed insignificant changes in the two varieties as affected by gamma irradiation and storage. A higher level of Ca, Cl, Fe and Na were detected in Silana as compared with corresponding contents in Daimont variety. Gamma irradiation treatments induced changes in DNA profile within the two varieties used.
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Keywords: potato, Solanum tuberosum, storage, minerals, phenols, TSS, DNA
American Journal of Food Science and Technology, 2013 1 (3),
Received September 11, 2013; Revised October 16, 2013; Accepted October 17, 2013Copyright © 2013 Science and Education Publishing. All Rights Reserved.
Cite this article:
- Hamideldin, N., and O.S. Hussien. "Morphological, Physiological and Molecular Changes in Solanum tuberosum L. in Response to Pre-sowing Tuber Irradiation by Gamma Rays." American Journal of Food Science and Technology 1.3 (2013): 36-41.
- Hamideldin, N. , & Hussien, O. (2013). Morphological, Physiological and Molecular Changes in Solanum tuberosum L. in Response to Pre-sowing Tuber Irradiation by Gamma Rays. American Journal of Food Science and Technology, 1(3), 36-41.
- Hamideldin, N., and O.S. Hussien. "Morphological, Physiological and Molecular Changes in Solanum tuberosum L. in Response to Pre-sowing Tuber Irradiation by Gamma Rays." American Journal of Food Science and Technology 1, no. 3 (2013): 36-41.
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Potato (Solanum tuberosum L.) is one of the unique and most potential crops having high productivity and supplementing major food requirement in the world after wheat, rice and maize. It constitutes nearly half of the worlds annual output of all root and tuber crops and has always remained in the top ten since last twenty years. Potatoes contain significant levels of important antioxidants, including phenolic compounds, flavonoids and carotenoids . It has high nutritive value and rich in vitamins A, B and C besides several minerals such as calcium, phosphorus and iron [2, 3]. Gamma rays belong to ionizing radiation and interact with atoms or molecules to produce free radicals in cells. These radicals can damage or modify important components of plant cells and have been reported to affect differentially the morphology, anatomy, biochemistry, and physiology of plants depending on the irradiation level [4, 5, 6]. Low doses of irradiation have been reported to stimulate plant growth . Radiation - induced mutations have been extensively used for improve crop plants. Studies conducted with several plant species indicated that, 2570 mutant have been obtained; among them, 1023 were produced via gamma irradiation . Molecular markers are important tools for precisely detecting the effects of gamma irradiation since they identify genetic polymorphisms at DNA level and have been used to study genetic dissimilarity in many crop species [9, 10, 11]. ISSR markers are highly polymorphic and are useful in studies on genetic diversity, phylogeny, gene tagging, genome mapping and evolutionary biology . Thus, this work aims to investigate the effect of γ- rays on morphological, physiological changes in plants as well as in the produced tubers and their DNA profile. The changes in the tubers after storage for 90 days were also investigated.
2. Materials and Methods
Two potato varieties (Daimont and Silana) were obtained from the Agriculture Research Center, Giza, Egypt. The two varieties, were irradiated with (0, 10 and 30 Gy), Irradiation facility used was Indian Gamma Cell Research Irradiator (60Co). The dose rate was 1Gy Sec-1. At the time of the experiment, irradiated and un-irradiated tubers were planted in sand- loamy soil in the farm, of the National Centre for Radiation Research and Technology (NCRRT), Cairo, Egypt. Soil fertilization was applied according to the recommendations of the Egyptian Ministry of Agriculture and Land Reclamation. The produced tubers were stored for 90 days at ambient room temperature. Changes in morphological, physiological and molecular parameters were investigated as affected by radiation and storage. The data recorded were:2.1. Morphological Changes
Plant height (cm), number of branches and leaves per plant, stem diameter, leaf area (cm2), and tuber diameter before and after storage (cm), tuber weight before and after storage (g) and tuber weight loss (g).2.2. Physiological Changes
2.2.1. Physical changes
The physical properties determined were the moisture content of tubers that was determined according to . Total soluble substances (TSS) were determined in a drop of potato juice, using refractometer, before and after storage according to .
2.2.2. Chemical Changes
The chemical properties determined were total phenol and mineral ratios. Total phenolic content of the extracts was determined using the Folin-Ciocalteu reagent according to . Methanolic extract (0.5 ml), 0.5 ml of Folin reagent, 10 ml of 7.5% sodium carbonate and deionized water were added to a final volume of 25 ml. After 1 h, the absorbance of the sample was measured at 725 nm against a blank by 02-120 shimadzu spectrophotometer. The results were expressed as mg gallic acid equivalent per gram of the fresh samples. Minerals were measured before storage in the NCRRT on Energy Dispersive X-Ray-Analysis Model: Oxford attached to a scanning electron microscope (JEOL-JSM 5400). Analysis depend on X-ray radiation emitted from each element-when the specimen is bombarded with high energetic electrons. That utilized to determine the kind of elements that exists in the specimen surface and their percentage. The elements estimated were calcium (Ca), chlorine (Cl), potassium (K), magnesium (Mg), sodium (Na) and phosphorus (P). The differences between the means were compared, using Duncan's multiple range tests .2.3. Molecular Characters
DNA was estimated by extraction and purification of sample using DNeasy mini Kit (QIAGEN). Inter simple sequence repeats (ISSRs) procedure (ISSR-PCR) reactions were conducted using five primers. The primer names and sequences are shown in Table 1.
Table 1. List of the primer names and their nucleotide sequences used in the study for ISSR procedure
Amplification was conducted in 25 µl reaction volume containing the following reagents: 2.5 µl of dNTPs (2.5 mM), 2.5 µl MgCl2 (2.5 mM), and 2.5 µl of 10 x buffer, 3.0 µl of Primer (10 pmol), 3.0 µl of template DNA (25 ng/ µl), 1 µl of Taq polymerase (1U/ µl) and 12.5 µl of sterile double distilled H2O . The PCRs were programmed for one cycle at 94ºC for 4 min followed by 45 cycles of 1 min at 94 ºC, 1 min at 57ºC, and 2 min at 72ºC. The reaction mixture was finally stored at 72ºC for 10 min. The PCR products were separated on a 1.5 % agarose gels and fragments sizes were estimated with the 100bp ladder marker (3000, 1500, 1000, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp). The similarity matrices were done using Gel works ID advanced software UVP-England Program.
3.Results3.1. Morphological Changes
Compared with non-irradiated control samples, the whole period of emergence was prolonged by 12-15 days for tubers treated with the dose 30 Gy of gamma rays. It was observed that 10 Gy dose promoted the emergence. No emergence occurred in Silana variety irradiated by 30 Gy dose. The data obtained in (Table 2) show that, in variety Silana, radiation increased plant height, stem diameter and tuber area but caused an inverse effect on most of parameters measured in Daimont variety.
Gamma irradiation had a pronounced effect on weight lose of tuber after storage. Weights lose decreased (about 50 g in Silana and 10g in Daimont) by the effect of gamma irradiation, as compared with un-irradiated tubers (Table 2). Weight losses were more obvious in un-irradiated Silana than in Daimont variety.3.2. Physical Changes
Data in Table 3 showed significant change in moisture content before and after storage due to radiation effects. Moisture content before storage increased significantly due to gamma irradiation in the two varieties (Silana and Daimont), while total soluble solids were not affected by irradiation in variety Salina before and after storage.
But, in Daimont variety total soluble solids were increased by gamma irradiation before storage.There was a slight decrease in moisture and total soluble solids in the irradiated and un-irradiated Daimont, whereas an increase in total soluble solids was obtained in irradiated and un-irradiated Salina.3.3. Chemical Changes
Total phenol content and mineral ratio was determined and shown in Table 4. γ-irradiation had non-significant effect on total phenols in the two varieties before and after storage. Total phenol content was increased in both potato varieties under investigation after storage. Mineral ratio were variable in the two varieties, Silana contained high levels of Ca and Fe. Daimont on the other hand, contained high levels of Cl, K, Mg, Na and P. Gamma irradiation increased Cl, K, Na and P contents and decreased Ca, Fe and Mg contents in variety Silana. In variety Daimont, gamma irradiation increased the contents of Ca, Cl, Fe, K and Na but decreased thos of Mg and P.3.4. DNA Polymorphism Using Inter Simple Sequence Repeats (ISSRs)
All the five primers used successfully amplified DNA fragments from potato DNA samples. The results indicated occurrence of structural changes in irradiated potato with the five primers: OP-A14, OP-A44, OP-A88, OP-B88 and OP-B98, respectively. A total of 45 fragments were visualized. Banding patterns for the used five random primers were scored as present (1) or absent (0) (Table 5). Genomic DNA polymorphisms due to irradiation are presented in Figure (1) for the five primers. In variety Silana, the percentage of polymorphism was 17.78%. The result of ISSR analysis refers to appearance of one band having a molecular size 700 bp with primer OP-A14, two bands ( 515 and 345 bp) with primer OP-A44, three bands with primer OP-A88 (730, 600 and 160bp) and one band with primer OP-B88 (240 bp) were appeared under gamma irradiation treatment by 10Gy. Meanwhile, with primer OP-B98, the band with molecular size 160 bp disappeared. In variety Daimont, the percentage of polymorphism in treatment with 10 Gy sample was 15.56% and was 26.67% in application of 30Gy) Gamma irradiation also induced the appearance of a band with primer OP-A14 having a molecular size 700 bp and caused disappearance of two bands (620 and 295 bp) under the effect of 10 and 30Gy. A band (120 bp) disappeared only at dose 30 Gy. In case of the primer OP-A44, a band (515bp) appeared in both irradiation doses. Gamma irradiation induced the disappearance of five bands with primer OP-A88; one of them (240 bp) by 10 Gy and the rest (540, 370, 180 and 120 bp) by 30 Gy. With primer OP-B98, one band (280 bp) disappeared under gamma irradiation and two bands appeared one having the molecular size 160 bp by 10 Gy and the other (630 bp) by 30 Gy.
The results obtained referred that potato tubers exposure to gamma irradiation before potato tubers sowing induced in morphological, physiological and molecular changes in two Solanum tuberosum L. varieties (Daimont and Silana). Also, it affected the produced tubers at harvest time and after storage. Gamma irradiation and storage effects differed in the two varieties used. Similar conclusions were also obtained by [18, 19] who noticed that the type of irradiation, dose rate or dose applied differently affected physiological parameters of different species, varieties, and cultivars. In the present study, gamma irradiation of tubers stimulated some morphological characters of the resulting plants. The exposure to low dose of γ-rays (10Gy) had stimulatory effect on specific morphological parameters and led to increased plants yield. Similar findings were found by [20, 21, 22]. The biological effect of γ-rays is based on the interaction with atoms or molecules in the cell, particularly water, to produce free radicals . These radicals can damage or modify important components of plant cells and have been reported to affect differentially the morphology, anatomy, biochemistry, and physiology of plants, depending on the radiation dose .
The physical characters of the two varieties studied in the present work varied in their response to gamma irradiation and storage. All results of physical characters evaluated are in consistent with  who observed differences between them in potato cultivars. Also, the effect of gamma irradiation on storage of the yielded tubers varied in the two varieties and these results are in agreement in with those of [25, 26]. They studied the effects of low doses of gamma rays and storage less than 120 days on potato cultivars and noticed insignificant changes on dry matter and phenol contents but weight loss increased with the increase in radiation doses. Radiation did not affect TSS in stored dragon fruits  and this concord with the present finding obtained with the two potato varieties. The previous studies indicated significant decrease in total soluble solids of potato tubers, insignificant decrease in total phenols and moisture content of potato tubers that exposed to 2.8 kGy of gamma rays . These effects included changes in the plant cellular structure and metabolism e.g., accumulation of phenolic compounds [4, 5, 29, 30] Phenolic contents increased more with storage time than with gamma irradiation dose [31, 32]. The increase in phenolic content might be due to dehydration that led to a higher concentration of solids at the end of the storage period. Additionally, stimulation of the synthesis of both antioxidants and polyphenols is known to occur with stress,which might have increased at the end of the storage period due to dehydration . Storage exerted a much greater influence on carotenoid content and phenolic content than did low-doses of gamma irradiation . Potato tubers contain several minerals that are important in the diet, including phosphorous, calcium, zinc, nitrogen and iron . Gamma irradiation induced changes in the mineral content of the two potato varieties. Molecular markers are important tools for precisely detecting the effects of gamma radiation since they identify genetic polymorphisms at DNA level and have been used to study genetic dissimilarity in many crop species [9, 11]. More polymorphism has been detected with the use of ISSRs than with any other assay procedure . In the present work, the polymorphic effect of gamma irradiation was depending on the applied dose and potato variety. In this respect variety Silana is more sensitive to gamma irradiation than variety Diamont. In case of Silana, the percentage of polymorphism was 17.78% on treatment with 10Gy and the 30Gy dose considered a lethal dose, where the tuber cannot germinate. The high dose of γ -irradiation is more effective than low in variety Diamont, the polymorphic percentage at dose 30Gy was 26.67% and 15.56% at 10Gy dose.
Table 5. DNA polymorphism using Inter simple sequence repeats (ISSRs) for two potato varieties Silana (S) and Daimont (D)
In variety Diamont, polymorphism percentage at dose 30Gy was 26.67% and at dose 10Gy was 15.56%. Gamma irradiation as a physical mutagen, is potent, inexpensive and easy to apply on the potato plantlets in vitro to create point mutations. The mutant plant variants can be easily selected from potato plants by RAPD-PCR . Nine potato unique markers could be identified among the 45 polymorphic bands, as analyzed by random amplified polymorphic DNA-polymerase chain reaction profiles . They detected one marker for the 5 Gy gamma dose and none for the 10 Gy and the highest number of markers (4) was obtained with the 2.5 Gy dose. Gamma rays induced molecules ionization to make apart free radicals these free radicals attacked to DNA molecule which make breaking one or two chains of DNA . Gamma irradiation resulted in appearance or disappearance of bands that can considered as molecular marker for radiation process .
Irradiation by low doses of γ-rays (≥ 10Gy) before sowing may be used as a valuable parameter to improve potato tubers yield and their shelf - life. It was observed that variety Silana is more sensitive to gamma irradiation than variety Diamont. The two bands with molecular size 700 and 515 bp at OP-A14 and OP-A44, respectively can be used as positive molecular markers for gamma radiation.
|||Brown, C.R., “Antioxidants in potato,” Am. J. Potato Res, 82.163-172. 2005.|
|||Dale, M.F., Griffiths, D.W. and Todd, D.T., “Effects of genotype, environment, and postharvest storage on the total ascorbate content of potato (Solanum tuberosum) tubers,”J Agric Food Chem, 51. 244-248. 2003.|
|In article||CrossRef PubMed|
|||Andre, C.M., Ghislain, M., Bertin, P., Oufir, M., Herrera, M.D., Hoffmann, L., Hausman. J.F., Larondelle, Y. and Evers, D., “Andean potato cultivars (Solanum tuberosum L.) as a source of antioxidant and mineral micronutrients,” J Agric Food Chem, 55. 366-378. 2007.|
|In article||CrossRef PubMed|
|||Kim, J. H., Baek, M. H., Chung, B. Y., Wi, S. G. and Kim, J. S., “Alterations in the photosynthic pigments and antioxidant machineries of red pepper (Capsicum annuum L.) seedlings from gamma-irradiated seeds,” J Plant Biol, 47. 314-321. 2004.|
|||Kova´cs, E. and Keresztes, A., “Effect of gamma and UV-B/C radiation on plant cell,” Micron, 33. 199–210. 2002.|
|||Wi, S. G., Chung, B. Y., Kim, J. H., Baek, M. H., Yang, D. H., Lee, J. W. and Kim, J. S., “Ultrastructural changes of cell organelles in Arabidopsis stem after gamma irradiation,” J Plant Biol, 48 (2). 195-200. 2005.|
|||Al-Safadi, B. and Simon, P.W., “The effects of gamma irradiation on the growth and cytology of carrot (Daucus carota L.) tissue culture,.” Environ Exp Bot, 30 (3). 361-371. 1990.|
|||FAO/IAEA, “Database of mutant variety and genetic stocks”, Available from URL, ww.mvd.iaea.org, 2009.|
|||Souframanien, J., Pawar, S.E. and Rucha, A.G., “Genetic variation in gamma ray induced mutants in blackgram as revealed by RAPD and ISSR markers”, Indian J Genet Plant Breed, 62. 291-305. 2002.|
|||Roy, A., Bandyopadhyay, A., Mahapatra, A.K., Ghosh, S.K., Singh, N.K. and Bansal, K.C., “Evaluation of genetic diversity in jute (Corchorus species) using STMS, ISSR and RAPD markers,” Plant Breed, 125(3).292-307. 2006.|
|||Barakat, M.N., Abdel Fattah, R.S., Badr, M. and El-Torky, M.G., “In vitro mutagenesis and identification of new variants via RAPD markers for improving Chrysanthemum morifolium,” Afr J Agric Res, 5(8).748-757. 2010.|
|||Reddy, M. P., Sarla, N. and Siddiq, E. A., “Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding,” Euphytica, 128. 9-17. 2002.|
|||AOAC, Official Methods of Analysis of the Association of Official Analytical Chemists 12th Ed. Washington DC U S A, 1975.|
|||AOAC, Official Methods of Analysis of the Association of Official Analytical Chemists 15th Ed. Arlington Virginia Washington DC U S A, 1990.|
|||Julkunen-Tiitto, R., “Phenolic constituents in the leaves of northern willows: methods for the analysis of certain phenolics,”J Agric. Food Chem, 33. 213-217 .1985.|
|||Duncan, Biometrics, 11.1-42. 1955.|
|||Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, A. Tingey, S. V., “DNA polymorphisms ampilified by arbitrary primers are useful as genetic markers,” Nucl Acids Res, 18.6531-6535.1990.|
|||Kim, J.H., Lee, M.H., Moon, Y.R., Kim, J.S., Wi, S.G., Kim, T.H. and Chung, B.Y., “Characterization of metabolic disturbances closely linked to the delayed senescence of Arabidopsis leaves after gamma -irradiation,” Environ Exp Bot, 67(2). 363-371. 2009.|
|||Zaka, R., Vandecasteele, C.M. and Misset, M.T., “Effect of low chronic doses of ionizing radiation on antioxidant enzymes and G6PDH activities in Stipa capillata (Poaceae),” J Exp Bot, 53(376). 1979-1987. 2002.|
|In article||CrossRef PubMed|
|||Maity, J.P., Mishra, D., Chakraborty, A., Saha, A., Santra, S.C. and Chanda, S., “Modulation of some quantitative and qualitative characteristics in rice (Oryza sativa L.) and mung (Phaseolus mungo L.) by ionizing radiation. Radiat Phys Chem, 74(5). 391-394. 2005.|
|||Yu, X., Wu, H., Wei, L.J., Cheng, Z., Xin, P., Huang, C., Zhang, K. and Sun,Y.Q., “Characteristics of phenotype and genetic mutations in rice after spaceflight,” Adv Space Res, 40 (4). 528-534. 2007.|
|||Melki, M. and Dahmani, T.H.,”Gamma irradiation effects on durum wheat (Triticum durum Desf) under various conditions,” Pak J Biol Sci, 12(23). 1531-1534.2009.|
|||Ashraf, M., Cheema, A. A., Rashid, M. and Qamar, Z., “Effect of γ-rays on M1 generation in basmati rice,” Pak. J. Bot, 35.791-795. 2003.|
|||Feltran, J. C., Lemos, L. B. and Vieites, R. L., “Technological quality and utilization of potato tubers,” Sci Agric (Piracicaba, Braz.), 61(6).598-603. 2004.|
|||Ezekiel, R., Singh, B. and Datta, P. S.,” Chipping quality of gamma irradiation potatoes of three Indian cultivars stored at 8, 12 and 16 ºC,” J Food Sci Techno Mysor, 45 (1). 36-43. 2008a.|
|||Ezekiel, R., Singh, B. and Datta, P. S., “Effect of low doses of gamma irradiation on the chipping quality of potatoes stored at 8 and 12 0 C,” Potato J, 35(1-2). 31-40. 2008b.|
|||Wall, M. M. and Khan, S. A., “Post harvest quality of dragon fruit (Hylocereus spp.) irradiation quarantine treatment”, Hort sci, 43.2115-2119. 2008.|
|||Abdalla, R..S., lethal effect of gamma radiation on some post harvest insects of certain fruit and vegetable crops. Bibilography ph D in Pesticides, 109-123. 2011.|
|||Wi, S.G., Chung, B.Y., Kim, J.S., Kim, J.H., Baek, M.H., Lee, J.W. and Kim, Y.S., “Effects of gamma irradiation on morphological changes and biological responses in plants,” Micron, 38(6). 553-564. 2007.|
|In article||CrossRef PubMed|
|||Ashraf, M., “Biotechnological approach of improving plant salt tolerance using antioxidants as markers”, Biotechnol Adv, 27(1).84-93. 2009.|
|||Zafrilla, P., Ferreres, F., Tomá.s-Barberán, F.A., “Effect of processing and storage on the antioxidant ellagic acid derivatives and flavonoids of red raspberry (Ru/nis idaeus) jams,” J Agric Food Chem, 49.3651-3655. 2001.|
|||Blessington, J.T., Miller., J. C., Nzararnba, M. N., Hale, A. L., Redivari, L., Scheuring, D.C. and Hallman, G. J., “The effects of low-dose gamma irradiation and storage time on carotenoids, antioxidant activity, and phenolics in the potato cultivar Atlantic,” Amer J of Potato Res, 84.125-131.2007.|
|||Kang, H. and Saltveit, M.E., “Antioxidant capacity of lettuce leaf tissue increases after wounding,” J Agric Food Chem, 50.7536-7541.2002.|
|||Yilmaz, G., Tuzen, M., Kandemir, N., Mendil, D. and Sari, H., “Trace metal levels in some modern cultivars and Turkish landraces of potato,” Asian J Chem, 17.79-84. 2005.|
|||Virk, P. S., Zhu, J., Newbury, H. J., Bryan, G. J., Jackson, M. T. and Ford-Lloyd, B. V., “Effectiveness of different classes of molecular marker for classifying and revealing variation in rice (Oryza sativa) germplasm,” Euphytica, 112. 275-284. 2000.|
|||Yaycil,i O. and Alikamanoğlu, S., “Induction of salt-tolerant potato (Solanum tuberosum L.) mutants with gamma irradiation and characterization of genetic variations via RAPD-PCR analysis,” Turk J Biol, 36. 405-412. 2012.|
|||Mahfouze, S.A., Esmael, A.M. and Mohasseb, H.A.A., “Genetic improvement of potato microtuber production in vitro by gamma irradiation, Biotecn Aplicada, 29.253-257. 2012.|
|||Jyoti, P.M., C.S.K. Sukalyan, P. Subrata, J. Jiin-Shuh, C. Alok, C. Anindita and C.S. Subhas.” Effects of gamma irradiation on edible seed protein, amino acids and genomic DNA during sterilization”. Food Chem.,114. 1237-1244. 2009.|
|||Hussein, O.S “Protein electrophoresis and DNA in herbs produced from irradiated Ambrosia maritima seeds grown under soil salinity and their resistance to insect” Am.j.plant Physiol.,7(6):261-268,2012.|