Figure 3. The effects of hydrogenized water on activation of transcription factor Nrf2 in human HepG2 cells. The cells were treated with a series of culture media containing various concentrations of hydrogenized water over night, and Nrf2 activation levels were determined as described in Materials and Methods. In this plot of relative Nrf2 activation level (compared to an untreated control) versus hydrogen concentration the EC₅₀ was calculated to be 0.097 mM H₂

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Hydrogenized Water Effects on Protection of Brain Cells from Oxidative Stress and Glutamate Toxicity

Robert Settineri, Jie Zhou, Jin Ji, Rita R. Ellithorpe, Steven Rosenblatt, Antonio Jimenez, Shigeo Ohta, Gonzalo Ferreira, Garth L. Nicolson

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