Figure 2. The effects of hydrogenized water on intracellular glutathione (GSH) levels in human SH-SY5Y neuroblastoma cells. The cells were treated with a series of culture media containing various concentrations of hydrogenized water for 24 hr prior to exposing to oxidative stress challenge, and GSH levels were determined as described in Materials and Methods. In this plot of GSH level (compared to an untreated control) versus hydrogen concentration the EC50 was calculated to be 0.0196 mM H2


Hydrogenized Water Effects on Protection of Brain Cells from Oxidative Stress and Glutamate Toxicity

Robert Settineri, Jie Zhou, Jin Ji, Rita R. Ellithorpe, Steven Rosenblatt, Antonio Jimenez, Shigeo Ohta, Gonzalo Ferreira, Garth L. Nicolson

American Journal of Food and Nutrition. 2018, 6(1), 9-13 doi:10.12691/ajfn-6-1-2