Figure 5. Puerarin attenuated TGF-β1-induced Smad expression in NRCMs NRCMs were transfected with 1 μL of Smad2siRNA. After 24 h of transfection, untransfected cells were treated with TGF-β1 (3 μg/L) individually or in combination with puerarin (1 g/L) for 24 h, and Smad2siRNA-transfected cells were treated with TGF-β1 (3 μg/L) for 24 h. Untreated cells were used as a control. mRNA (A) and protein (B) expression of Smad2, pSmad2, and total Smad2/3 were determined using qRT-PCR and Western blot analysis, respectively. (C) Quantification of (B). Data are expressed as mean ± SD. *P < 0.05 vs. control; #P < 0.01 vs. TGF-β1 group; &P < 0.05 vs. puerarin group (n=6)

From

Puerarin Attenuates Transforming Growth Factor Beta-1 Induced Hypertrophic Responses and Smad Proetin Upregulation in Neonatal Rat Cardiomyocytes

Yongying Shi, Aijun Liu, Junjiang Lu, Guangyuan Chen, Shiming Liu, Minsheng Chen, Chengfeng Luo

American Journal of Biomedical Research. 2020, 8(2), 40-46 doi:10.12691/ajbr-8-2-3