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From
Cicada Shells as Chemical Education Resources to Study Functional Groups and Environmental Cycles
Naoki Maruyama, Toratane Munegumi
World Journal of Chemical Education
.
2023
, 11(4), 127-133 doi:10.12691/wjce-11-4-1
Figure 1.
Acid-mediated hydrolysis of chitin through the formation of hemiacetal and free amino moieties to give glucosamine.
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Fig
ure
2.
Benedict reaction with reducing sugars
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ure
3.
Ninhydrin reaction with compounds containing amino groups.
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Fig
ure
4.
Dissection of cicada shells for the acid-hydrolysis reaction
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Figure
5.
Benedict reaction of (a) standard chitin and (b) cicada shells hydrolyzed in 6 M HCl at 110 C. Using Benedict: 0.50 mL for 24 h (A); 1.00 mL for 24 h (B).
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Figure 6
. Benedict reaction of (a) standard chitin and (b) cicada shells hydrolyzed in 12 m HCl at 110 C. (c) Acid-hydrolysis of cicada shells in 12 m HCl at 110 C before the Benedict reaction. 0 h (A): without chitin; 0 h (B): with chitin.
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Figure 7.
Benedict reaction of standard (a) chitin and (b) cicada shells hydrolyzed in 12 M HCl at 25 C.
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Figure 8
. Ninhydrin reaction with the reaction mixtures of hydrolyzed chitin and cicada shells in 6 M HCl at 110 C.
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Figure 9.
Ninhydrin reaction with the reaction mixtures of hydrolyzed chitin and cicada shells in 12 M HCl at 110 C
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Figure 10.
Ninhydrin reaction with reaction mixtures of hydrolyzed chitin in 12 M HCl at 25 C
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Figure 11.
Ninhydrin reaction with reaction mixtures of hydrolyzed cicada shells in 12 M HCl at 25C
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Figure 12.
An example of the gel generated from cicada shells after dialysis against 12,000-14,000 Da molecular weight cutoff.
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Figure 13.
Metabolic pathway from glycogen and amino acid to chitin. Enzyme names are omitted from the metabolic paths and cycles.
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