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Can Fucoidan Decrease the Mortalities Caused by Columnaris Disease in Nile Tilapia?

Hebatallah Ahmed Mahgoub

World Journal of Agricultural Research. 2018, 6(1), 1-4 doi:10.12691/wjar-6-1-1
  • Figure 1. Isolation of Flavobacterium columnare on selective medium (cytophaga agar). A) Colonies on cytophaga agar are flat, rhizoid, strongly adherent, and spread across solid media surfaces forming irregular margins. B) A higher magnification of one of Flavobacterium columnare rhizoid colonies
  • Figure 2. PCR amplification products of Flavobacterium columnare 16S ribosomal DNA using Flavobacterium columnare specific primers. Amplification products (250 bp) were resolved through a 2% agarose gel. Lane 1: 50 bp ladder (iNtTRON). Lane 2: Flavobacterium columnare isolated from the muscle. Lane 3: Flavobacterium columnare isolated from the tail fin. Lane 4: Flavobacterium columnare isolated from the gills
  • Figure 3. Gross examination of Nile tilapia infected with Columnaris disease, pre- and post-treatment with fucoidan. A) An infected fish showing an ulcerated and haemorrhagic external surface (1) and eroded tai-fin (2). B) An infected fish showing pale gills (3). C) An infected fish showing body stiffness/rigidity and eroded tai-fin (2). D) An infected treated fish showing normal external surface, body figure/elasticity, and fins
  • Figure 4. Miscroscopical alterations caused by Columnaris disease infection in Nile tilapia. A) The micrograph shows marked intermuscular leukocytic infiltration, fibroblast proliferation, and extravasation of erythrocytes. B) The micrograph shows partial muscle degradation or myomalacia. C) The micrograph shows partial and complete muscle degradation or myomalacia. D) The micrograph shows intermuscular oedema
  • Figure 5. Tissue damage score of the surface muscle in different experimental groups. Score data were analysed using one-way ANOVA, followed by Tukey’s multiple comparison test, where each group was compared to the control group and every other group. **** means p value < 0.0001