Figure 2. Effect of pH on the reaction of D-PA with NBD-CL. D-PA (15 µg/ml): 1mL; buffer ; 1.0ml; temperature 25ºC; reaction time: 25min

From

Development and Validation of Spectrophotometric Method for Determination of Penicillamine (PA) in Pharmaceutical Formulation Using 4-Choro-Nitrobenzo-2-Oxa-1, 3-Diazol (NBD-CL)

Abdalla Ahmed Elbashir, Amira Anwar Babikir Alfadil

World Journal of Analytical Chemistry. 2013, 1(2), 18-22 doi:10.12691/wjac-1-2-1