Figures index

From

Transformation of Iranian Melon for Increasing Resistance to Fungal Diseases

Mohammad Akbari, Esmaeil Nadaf, Mahmoud Lotfi, Masoud Tohidfar

Research in Plant Sciences. 2013, 1(1), 1-3 doi:10.12691/plant-1-1-1
  • Figure 1. Transfer explants on selector medium: 1) inoculated explants with Agrobacterium 2) The transgenic explants left green and non-transgenic explants have been yellow in kanamycin medium 3) control explants after a while without regeneration became necrosis
  • Figure 2. Direct regeneration of shoots from leaf cotyledonary explants on selective medium: 1) generate a new shoot buds of the inoculated explants 2) desirable transgenic seedlings remained green and Some unfavorable transgenic got yellow and lost 3) growth of resistant shoot in selective medium with more kanamycin (50mg/l) 4) remove of non-transgenic escaped shoot 5) growth of resistant shoots in elongation medium 6) kanamycin-resistant shoots elongation
  • Figure 3. Electrophoresis product of PCR reaction for gene of chitinase (A) and β-1, 3 glucanase (B), agarose gel 1%: L) DNA ladder (1kb) 2) DNA plasmid pBI121 3) DNA genome of non-transgenic plant (control) 4) DNA genome of transgenic plants 5) negative control (water).