Figure 5. Effects of ethanol extracts of napiergrass Taishigrass no. 2 (NT2) on mRNA expressions of cluster of differentiation 14 (CD14) (A) and Toll-like receptor 4 (TLR4) (B) by RAW 264.7 macrophages. Cells were treated with various concentrations of the extracts in the presence of lipopolysaccharide (LPS) (100 ng/ml) for 24 h. Total cellular RNA was isolated and analyzed for mRNA expression with a real-time PCR. The relative gene expression was obtained after normalization with GAPDH. Data are expressed as the mean ± SD (n = 3). ab, data that share no letters significantly differ by a one-way ANOVA (p < 0.05). TLR4, p > 0.05 : Suppression of Inflammatory Mediators by Ethanol Extracts of Pennisetum purpureum S. (Napiergrass Taishigrass no. 2) in Activated RAW 264.7 Macrophages : Science and Education Publishing

Figure 5. Effects of ethanol extracts of napiergrass Taishigrass no. 2 (NT2) on mRNA expressions of cluster of differentiation 14 (CD14) (A) and Toll-like receptor 4 (TLR4) (B) by RAW 264.7 macrophages. Cells were treated with various concentrations of the extracts in the presence of lipopolysaccharide (LPS) (100 ng/ml) for 24 h. Total cellular RNA was isolated and analyzed for mRNA expression with a real-time PCR. The relative gene expression was obtained after normalization with GAPDH. Data are expressed as the mean ± SD (n = 3). ab, data that share no letters significantly differ by a one-way ANOVA (p < 0.05). TLR4, p > 0.05

Journal of Food and Nutrition Research. 2020, 8(8), 392-398 doi:10.12691/jfnr-8-8-2