Figure 4. Effects of ethanol extracts of napiergrass Taishigrass no. 2 (NT2) on matrix metalloproteinase (MMP) activities in RAW 264.7 macrophages. Cells were treated with different concentrations of extracts in the absence or presence of lipopolysaccharide (LPS) (100 ng/ml) for 24 h. The medium was collected to analyze MMP activities by gelatin zymography (A), and clear bands were quantified by Image-Pro Plus software (B, C). mRNA expression of MMP-9 was determined by a real-time PCR (D). Data are expressed as the mean ± SD from three independent experiments. a,b,#,*, data that share no symbols significantly differ within either LPS-untreated or LPS-treated groups by a one-way ANOVA (p < 0.05). MMP-2, p >0.05 : Suppression of Inflammatory Mediators by Ethanol Extracts of Pennisetum purpureum S. (Napiergrass Taishigrass no. 2) in Activated RAW 264.7 Macrophages : Science and Education Publishing

Figure 4. Effects of ethanol extracts of napiergrass Taishigrass no. 2 (NT2) on matrix metalloproteinase (MMP) activities in RAW 264.7 macrophages. Cells were treated with different concentrations of extracts in the absence or presence of lipopolysaccharide (LPS) (100 ng/ml) for 24 h. The medium was collected to analyze MMP activities by gelatin zymography (A), and clear bands were quantified by Image-Pro Plus software (B, C). mRNA expression of MMP-9 was determined by a real-time PCR (D). Data are expressed as the mean ± SD from three independent experiments. a,b,#,*, data that share no symbols significantly differ within either LPS-untreated or LPS-treated groups by a one-way ANOVA (p < 0.05). MMP-2, p >0.05

Journal of Food and Nutrition Research. 2020, 8(8), 392-398 doi:10.12691/jfnr-8-8-2