Figure 3. Effects of ethanol extracts of napiergrass Taishigrass no. 2 (NT2) on the production of interleukin (IL)-6 (A) and tumor necrosis factor (TNF)-α (B) by RAW 264.7 macrophages. Cells were treated with different concentrations of extracts, in the presence or absence of lipopolysaccharide (LPS) (100 ng/ml), for 24 h. Secretion of IL-6 and TNF-α into the cultured medium was analyzed by commercial assay kits according to the manufacturers’ instructions. Data are expressed as the mean ± SD from three independent experiments. * Significantly differs from the 0 (ethanol vehicle control) by Student’s t-test. abc, data that share no letters significantly differ by a one-way ANOVA (p < 0.05) : Suppression of Inflammatory Mediators by Ethanol Extracts of Pennisetum purpureum S. (Napiergrass Taishigrass no. 2) in Activated RAW 264.7 Macrophages : Science and Education Publishing

Figure 3. Effects of ethanol extracts of napiergrass Taishigrass no. 2 (NT2) on the production of interleukin (IL)-6 (A) and tumor necrosis factor (TNF)-α (B) by RAW 264.7 macrophages. Cells were treated with different concentrations of extracts, in the presence or absence of lipopolysaccharide (LPS) (100 ng/ml), for 24 h. Secretion of IL-6 and TNF-α into the cultured medium was analyzed by commercial assay kits according to the manufacturers’ instructions. Data are expressed as the mean ± SD from three independent experiments. * Significantly differs from the 0 (ethanol vehicle control) by Student’s t-test. abc, data that share no letters significantly differ by a one-way ANOVA (p < 0.05)

Journal of Food and Nutrition Research. 2020, 8(8), 392-398 doi:10.12691/jfnr-8-8-2