Figure 2. Effects of ethanol extracts of napiergrass Taishigrass no. 2 (NT2) on nitric oxide (NO) production (A) and inducible NO synthase (iNOS) protein expression (B) by RAW 264.7 macrophages. Cells were treated with different concentrations of extracts, in the presence or absence of lipopolysaccharide (LPS) (100 ng/ml), for 24 h. NO production was measured by the Griess reaction. iNOS protein expression was determined by Western blotting and the relative expressions were normalized to β-actin. Data are expressed as the mean ± SD from three independent experiments. * Significantly differs from the 0 (ethanol vehicle control) by Student’s t-test. ab, data that share no letters significantly differ by a one-way ANOVA (p < 0.05) : Suppression of Inflammatory Mediators by Ethanol Extracts of Pennisetum purpureum S. (Napiergrass Taishigrass no. 2) in Activated RAW 264.7 Macrophages : Science and Education Publishing

Figure 2. Effects of ethanol extracts of napiergrass Taishigrass no. 2 (NT2) on nitric oxide (NO) production (A) and inducible NO synthase (iNOS) protein expression (B) by RAW 264.7 macrophages. Cells were treated with different concentrations of extracts, in the presence or absence of lipopolysaccharide (LPS) (100 ng/ml), for 24 h. NO production was measured by the Griess reaction. iNOS protein expression was determined by Western blotting and the relative expressions were normalized to β-actin. Data are expressed as the mean ± SD from three independent experiments. * Significantly differs from the 0 (ethanol vehicle control) by Student’s t-test. ab, data that share no letters significantly differ by a one-way ANOVA (p < 0.05)

Journal of Food and Nutrition Research. 2020, 8(8), 392-398 doi:10.12691/jfnr-8-8-2