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Chlorogenic Acid Decreases Lipid Accumulation in 3T3-L1 Adipocytes by Modulating the Transcription Factors

Ching-Chih Liu, Jen-Yin Chen, Chin-Chen Chu, Shih-Ying Chen, Heuy-Ling Chu, Pin-Der Duh

Journal of Food and Nutrition Research. 2020, 8(7), 313-319 doi:10.12691/jfnr-8-7-2
  • Figure 1. Effect of chlorogenic acid (CA) on cell proliferation in 3T3-L1 pre-adipocytes and adipocytes. (A) 3T3-L1 pre-adipocytes were treated with CA for 24 h. (B) 3T3-L1 adipocytes were harvested 8 days after the initiation of differentiation. The cells were treated with chlorogenic acid for 24 h. Data are expressed as the mean ± SD of three independent experiments. *(p <0.05), compared to the control
  • Figure 2. Effect of chlorogenic acid (CA) on adipogenesis. (A) The microscopic images of Oil red O-staining of CA-treated 3T3-L1 cells. (B) Oil red O staining of EECF-treated 3T3-L1 cells. 3T3-L1 cells were incubated with chlorogenic acid (CA) for 24 h at 37°C in 5 % CO2 incubator. (C) CA-mediated suppression of intracellular triglyceride levels of 3T3-L1 cells. 3T3-L1 cells were incubated with CA for 24 h at 37°C in 5 % CO2 incubator. Data are expressed as the mean ± SD of three independent experiments. *(p <0.05), compared to the control
  • Figure 3. Effect of chlorogenic acid (CA) on GPDH activity (A) and PPARγ protein expression (B) protein expression in 3T3-L1 cells. (A) The cells were incubated with CA for 24 h at 37°C in 5 % CO2 incubator. (B) The cells were incubated with CA for 4 h at 37°C in 5 % CO2 incubator. Data are expressed as the mean ± SD of three independent experiments. *(p <0.05), compared to the control
  • Figure 4. Effect of chlorogenic acid (CA) on expression of adipogenic genes in 3T3-L1 adipoctyes. (A) The effect of CA on gene levels of PPARγ in 3T3-L1 cells. The cells were incubated with CA for 2 h. (B) The effect of CA on gene levels of C/EBPα in 3T3-L1 cells. The cells were incubated with CA for 2 h. (C) The effect of CA on gene levels of SREBP-1c in 3T3-L1 cells. The cells were incubated with CA for 2 h. (D) The effect of CA on gene levels of FAS in 3T3-L1 cells. The cells were incubated with CA for 2 h. Data are expressed as the mean ± SD of three independent experiments. *(p <0.05), compared to the control