Figure S1. The representative HPLC chromatogram of catechin compounds in high-temperature-processed green tea extract (HTP_GTE). HTP_GTE was standardized using reverse-phase high-performance liquid chromatography (HPLC)-PDA (Alliance 2695 system, Waters) system. Separation was carried out using a Thermo Syncronis C18 column (250 × 4.6 mm, I.D., 5 μm; Thermo Fisher Scientific Inc.). The mobile phases were 0.1% acetic acid in water for solvent A and acetonitrile for solvent B. The gradient elution was 90% A + 10% B at 0-10 min, 85% A + 15% B at 10-30 min, 80% A + 20% B at 30-53 min, 5% A + 95% B at 53 -55 min, 90% A + 10% B at 55-60 min with a flow rate of 1.0 mL/min. The injection volume of sample was 20 µl, and the wavelength of UV was 280 nm. Each peak was named by corresponding compound obtained by standard curve. EGCG, (-)-epigallocatechin 3-O-gallate; GCG, (-)-gallocatechin 3-O-gallate; EGC, (-)-epigallocatechin; GC, (-)-gallocatechin; ECG, (-)-epicatechin 3-O-gallate; CG, (+)-catechin 3-O-gallate; EC, (-)-epicatechin; C, catechin : Effect of Epimerized Catechins-Rich Green Tea Extract on Global Cognitive Function in Healthy Individuals: A Pilot Study : Science and Education Publishing

Figure S1. The representative HPLC chromatogram of catechin compounds in high-temperature-processed green tea extract (HTP_GTE). HTP_GTE was standardized using reverse-phase high-performance liquid chromatography (HPLC)-PDA (Alliance 2695 system, Waters) system. Separation was carried out using a Thermo Syncronis C18 column (250 × 4.6 mm, I.D., 5 μm; Thermo Fisher Scientific Inc.). The mobile phases were 0.1% acetic acid in water for solvent A and acetonitrile for solvent B. The gradient elution was 90% A + 10% B at 0-10 min, 85% A + 15% B at 10-30 min, 80% A + 20% B at 30-53 min, 5% A + 95% B at 53 -55 min, 90% A + 10% B at 55-60 min with a flow rate of 1.0 mL/min. The injection volume of sample was 20 µl, and the wavelength of UV was 280 nm. Each peak was named by corresponding compound obtained by standard curve. EGCG, (-)-epigallocatechin 3-O-gallate; GCG, (-)-gallocatechin 3-O-gallate; EGC, (-)-epigallocatechin; GC, (-)-gallocatechin; ECG, (-)-epicatechin 3-O-gallate; CG, (+)-catechin 3-O-gallate; EC, (-)-epicatechin; C, catechin

Journal of Food and Nutrition Research. 2020, 8(3), 139-148 doi:10.12691/jfnr-8-3-4