Figure 2. Involvement of FOXO3 to mediate SOD2 expression by daidzein. A) Protein from HK-2 cell treated with daidzein was prepared by the cytosol and nucleous fractional method described in material and method section. Then, FOXO3 and SOD2 expression level was analyzed by Western blotting. β-actin was used for cytosol loading control and PARP1 was for nucleous loading control. B) Immunofluorescence analysis of FOXO3 in HK-2 cells treated with daidzein was performed by the method described in the methods. C) siRNA against FOXO3 was transfected into HK-2 cells followed by daidzein treatment. Then, FOXO3 and SOD2 expression level was analyzed by Western blotting. β-actin was used for loading control D) siRNA against FOXO3 was transfected into HK-2 cells followed by daidzein treatment. Then, ROS level in HK-2 cells was measured by ROS detection kit

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Daidzein Has Anti-Oxidant Activity in Normal Human Kidney Tubular HK-2 Cells via FOXO3/SOD2 Pathway

Jongsung Lee, See-Hyoung Park

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