Figure 5. Effect of nsLTP2 on VCAM-1 expression in HUVECs treated with TNF-α in the presence or absence of HO-1 inhibitors. HUVECs were treated with 10 nM nsLTP in the presence or absence of HO-1 inhibitors (10nM ZnPP IX and SnPP IX) and subsequently treated with 20ng/mL TNF-α. Cell lysates were subjected to SDS-PAGE and Western blotting analysis of VCAM protein expression level. Densitometric analysis of VCAM expression normalized to actin is expressed as % of stressed cells and was carried out with the ChemiDoc MP System (BIORAD). Data represent means ± SD of three independent experiments. §p < 0.05, compared with control HUVECs, *p < 0.05, compared with HUVECs stressed by TNF-α. : Protective Effect of Wheat Derived Non-specific lipid-transfer Protein 2 on Vascular Endothelium Inflammation : Science and Education Publishing

Figure 5. Effect of nsLTP2 on VCAM-1 expression in HUVECs treated with TNF-α in the presence or absence of HO-1 inhibitors. HUVECs were treated with 10 nM nsLTP in the presence or absence of HO-1 inhibitors (10nM ZnPP IX and SnPP IX) and subsequently treated with 20ng/mL TNF-α. Cell lysates were subjected to SDS-PAGE and Western blotting analysis of VCAM protein expression level. Densitometric analysis of VCAM expression normalized to actin is expressed as % of stressed cells and was carried out with the ChemiDoc MP System (BIORAD). Data represent means ± SD of three independent experiments. ^§p < 0.05, compared with control HUVECs, *p < 0.05, compared with HUVECs stressed by TNF-α.

Journal of Food and Nutrition Research. 2018, 6(6), 386-392 doi:10.12691/jfnr-6-6-6