Figure 4. Effect of nsLTP2 (LTP2), Atorvastatin and Simvastatin on HO-1 protein expression level. HUVECs were treated with 10 nM nsLTP2 (LTP2), 10 nM - 50 µM Atorvastatin (A) or 10 nM - 50 µM Simvastatin (S) for 24 h. Cell lysates were subjected to SDS-PAGE and Western blotting analysis of HO-1 protein expression level. Densitometric analysis of HO-1 expression, normalized to actin, is expressed as fold increase over control and was carried out with the ChemiDoc MP System (BIORAD). Data represent means ± SD of three independent experiments. §p < 0.05, compared with control HUVECs : Protective Effect of Wheat Derived Non-specific lipid-transfer Protein 2 on Vascular Endothelium Inflammation : Science and Education Publishing

Figure 4. Effect of nsLTP2 (LTP2), Atorvastatin and Simvastatin on HO-1 protein expression level. HUVECs were treated with 10 nM nsLTP2 (LTP2), 10 nM - 50 µM Atorvastatin (A) or 10 nM - 50 µM Simvastatin (S) for 24 h. Cell lysates were subjected to SDS-PAGE and Western blotting analysis of HO-1 protein expression level. Densitometric analysis of HO-1 expression, normalized to actin, is expressed as fold increase over control and was carried out with the ChemiDoc MP System (BIORAD). Data represent means ± SD of three independent experiments. §p < 0.05, compared with control HUVECs

Journal of Food and Nutrition Research. 2018, 6(6), 386-392 doi:10.12691/jfnr-6-6-6