Figure 1. Effect of nsLTP2 on cell adhesion molecule expression in HUVECs treated with TNF-α. After 10 nM nsLTP2 (LTP2) pre-treatment for 24 h, HUVECs were stressed with 20 ng/mL TNF-α. Cell lysates were subjected to SDS-PAGE and Western blotting analysis, using primary antibodies directed to VCAM-1, ICAM-1. Images show a representative experiment of three independent analyses. Densitometric analysis of bands intensity, normalized to actin, was expressed as % of stressed cells and was carried out with the ChemiDoc MP System (BIORAD). Data represent means ± SD of three independent experiments. §p < 0.05, vs control HUVECs; *p < 0.05, vs HUVECs stressed with TNF-α : Protective Effect of Wheat Derived Non-specific lipid-transfer Protein 2 on Vascular Endothelium Inflammation : Science and Education Publishing

Figure 1. Effect of nsLTP2 on cell adhesion molecule expression in HUVECs treated with TNF-α. After 10 nM nsLTP2 (LTP2) pre-treatment for 24 h, HUVECs were stressed with 20 ng/mL TNF-α. Cell lysates were subjected to SDS-PAGE and Western blotting analysis, using primary antibodies directed to VCAM-1, ICAM-1. Images show a representative experiment of three independent analyses. Densitometric analysis of bands intensity, normalized to actin, was expressed as % of stressed cells and was carried out with the ChemiDoc MP System (BIORAD). Data represent means ± SD of three independent experiments. ^§p < 0.05, vs control HUVECs; *p < 0.05, vs HUVECs stressed with TNF-α

Journal of Food and Nutrition Research. 2018, 6(6), 386-392 doi:10.12691/jfnr-6-6-6