Figure 3. Inhibitory effects of CFE administration on mRNA expression of iNOS and COX-2. Raw264.7 cells (1.0×106 cells/mL) were pre-incubated for 2 h with F. erecta CH2Cl2 fractions (CFE; 0, 5, 10 and 20 µg/mL). Cells were then stimulated with LPS (1 μg/mL) for 24 h. The LPS-induced changes in the mRNA levels of iNOS and COX-2 were determined by PCR. β-actin was used as a loading control. Data are mean ± standard deviation (SD). The value of the LPS alone group was set at 1, and results were expressed as a ratio relative to the LPS alone group. *P<0.05 and **P<0.01 (test fraction vs. the LPS alone group). LPS, lipopolysaccharide; iNOS, inducible nitric oxide synthase; COX-2, cyclooxygenase-2 : The CH2Cl2 Extract Fraction from Ficus erecta var. sieboldii (Miq.) King Suppresses Lipopolysaccharide-mediated Inflammatory Responses in Raw264.7 Cells : Science and Education Publishing

Figure 3. Inhibitory effects of CFE administration on mRNA expression of iNOS and COX-2. Raw264.7 cells (1.0×10⁶cells/mL) were pre-incubated for 2 h with F. erecta CH₂Cl₂ fractions (CFE; 0, 5, 10 and 20 µg/mL). Cells were then stimulated with LPS (1 μg/mL) for 24 h. The LPS-induced changes in the mRNA levels of iNOS and COX-2 were determined by PCR. β-actin was used as a loading control. Data are mean ± standard deviation (SD). The value of the LPS alone group was set at 1, and results were expressed as a ratio relative to the LPS alone group. ^*P<0.05 and ^**P<0.01 (test fraction vs. the LPS alone group). LPS, lipopolysaccharide; iNOS, inducible nitric oxide synthase; COX-2, cyclooxygenase-2

Journal of Food and Nutrition Research. 2018, 6(6), 356-364 doi:10.12691/jfnr-6-6-2