Figures index

From

Leucine Exerts Lifespan Extension and Improvement in Three Types of Stress Resistance (Thermotolerance, Anti-Oxidation and Anti-UV Irradiation) in C. elegans

Hongyuan Wang, Jin Wang, Zhizhou Zhang

Journal of Food and Nutrition Research. 2018, 6(10), 665-673 doi:10.12691/jfnr-6-10-8
  • Figure 1. Leucine extends lifespan significantly in C. elegans. (A) Chemical structure of L-Leucine. (B) Survival curves of wild-type nematodes treated with 0 (black), 100μM (red), 1000μM (blue) and 10000μM (green) Leu
  • Figure 2. Leu-treated worms exert changes in body length and brood size. (A) Body length of nematodes treated with 0 (black), 100μM (red), 1000μM (blue) and 10000μM (green) Leu. (B) Brood size of nematodes treated with 0 (black), 100μM (red), 1000μM (blue) and 10000μM (green) Leu. Multiple t-test was used for both experiments. *p<0.05, **p<0.01.
  • Figure 3. Leu increased stress resistance in C. elegans. (A) Survival curve (left) and proportion analysis of average motility (right) of nematodes treated with 0 (black), 100μM (red), 1000μM (blue) and 10000μM (green) Leu under heat shock condition. (B) Survival curve (left) and proportion analysis of average motility (right) of nematodes treated with 0 (black), 100μM (red), 1000μM (blue) and 10000μM (green) Leu under paraquat in NGM condition. (C) Survival curve (left) and proportion analysis of average motility (right) of nematodes treated with 0 (black), 100μM (red), 1000μM (blue) and 10000μM (green) Leu under UV irradiation condition
  • Figure 4. Lifespan extension induced by supplementary Leu is dependent on IIS signaling pathway. (A) Survival curve of daf-2 mutant of nematodes treated with 0 (black) and 1000μM (blue) Leu. (B) Survival curve of daf-16 mutant of nematodes treated with 0 (black) and 1000μM (blue) Leu. (C) mRNA expression of DAF-16 analyzed by quantitative real-time PCR of worms treated with 0 (black) and 1000μM (blue) Leu. *p<0.05.