Figure 4. Lifespan extension induced by supplementary Leu is dependent on IIS signaling pathway. (A) Survival curve of daf-2 mutant of nematodes treated with 0 (black) and 1000μM (blue) Leu. (B) Survival curve of daf-16 mutant of nematodes treated with 0 (black) and 1000μM (blue) Leu. (C) mRNA expression of DAF-16 analyzed by quantitative real-time PCR of worms treated with 0 (black) and 1000μM (blue) Leu. *p<0.05.

From

Leucine Exerts Lifespan Extension and Improvement in Three Types of Stress Resistance (Thermotolerance, Anti-Oxidation and Anti-UV Irradiation) in C. elegans

Hongyuan Wang, Jin Wang, Zhizhou Zhang

Journal of Food and Nutrition Research. 2018, 6(10), 665-673 doi:10.12691/jfnr-6-10-8