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From

Anti-inflammatory Effects of Extract from Eucommia ulmoides Oliv. Leaves on Macrophage Cells

Hyun Woo Kang

Journal of Food and Nutrition Research. 2017, 5(5), 309-312 doi:10.12691/jfnr-5-5-4
  • Figure 1. Effect of extract of EUO and/or LPS on the cell viability of RAW264.7 cells using the MTT assay. RAW264.7 cells were incubated with 0.1, 1, 5, and 10 μg/mL of extract and LPS (100 ng/mL) for 24 h. The results are shown as percentages of control samples (A). Effects of extract of EUO on NO production. RAW264.7 cells were pre-treated with the indicated concentrations of extract (0.1, 1, 5, and 10 μg/mL) for 30 min before incubating with LPS (100 ng/mL) for 24 h (B). Data are presented as the mean ± S.E.M. (n = 3) for three independent experiments
  • Figure 2. Effect of extract of EUO on LPS-induced iNOS (A) and COX-2 (B) protein expression in RAW264.7 cells. Cell lysates were electrophoresed and the protein expression of iNOS and COX-2 was detected by the specific antibody. β-actin was used as an internal control for the western blot analysis. Quantification data are shown in the lower panel. Data are presented as the mean ± S.E.M. (n = 3) for three independent experiments