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Ecklonia cava-derived Polysaccharide Prevent Hydro Peroxide-induced Oxidative Stress and Neurotoxicity in Human Microglial HMO6 Cells

Yeon-Joo Lee, Ji-Hyun Hwang, Kui-Jin Kim, Boo-Yong Lee

Journal of Food and Nutrition Research. 2017, 5(3), 187-190 doi:10.12691/jfnr-5-3-8
  • Figure 1. Effect of ECAP on cellular markers of apoptosis in HMO6 cells. Cells were treated with indicated concentrations of ECAP for 6 hours before treatment with 500 μM hydro peroxide for 6 hours. (A) Western blot analysis for detecting the levels of p53, cleaved caspase-3, and Bcl-2. (B) The expression levels of p53, caspase-3, and Bcl-2 mRNA were assessed by RT-PCR
  • Figure 2. ECAP regulates hydro peroxide-mediated oxidative stress-associated proteins and mRNA levels in HMO6 cells. (A) Western blot were performed by incubating total lysates were with G6PDH, NOX4, and α-tubulin specific antibodies. (B) The expression levels of NOX4, G6PDH, and α-tubulin mRNA were evaluated by RT-PCR
  • Figure 3. ECAP suppressed the hydro peroxide-mediated alteration of anti-oxidant enzymes including SOD1, SOD2, and catalase in HMO6 cells. Total cellular protein was extracted and subjected to SDS-PAGE. (A) Western blots are shown for antioxidant enzymes such as SOD1, SOD2, and catalase. (B) The expression levels of SOD1, SOD2, and catalase mRNA were evaluated by RT-PCR