Figure 3. Induction of proinflammatory cytokines in RAW264.7 cells and mouse primary splenocytes by PTE10. Cytokine expression was tested in RAW264.7 cells during immune stimulation by PTE10. After PTE10 treatment, cytokines expression was increased. (A) IL-1β expression in mouse primary splenocytes. (B) IL-2 expression in mouse primary splenocytes. (C) IL-4 expression in mouse primary splenocytes. (D) IFN-γ expression in mouse primary splenocytes. (E) iNOS expression in mouse primary splenocytes. (F) phosphorylated-Akt, phosphorylated-JNK, phosphorylated-ERK expression was detected by Western blot analysis with PTE10 treatment. *P < 0.05 and **P < 0.01 compared to control

From

Porphyra tenera Extracts Have Immune Stimulation Activity via Increasing Cytokines in Mouse Primary Splenocytes and RAW264.7 Macrophages

Hee-Bum Kang, Ji-Hye Song, Yanghee You, Jeongjin Park, Sungmin Kwak, Yoo-Hyun Lee, Jeongmin Lee, Su-il Kim, Kyung-Chul Choi, Woojin Jun

Journal of Food and Nutrition Research. 2016, 4(9), 558-565 doi:10.12691/jfnr-4-9-1