Figure 2. Effect of rutin on LPS-induced expression of COX-2 (A) and iNOS (B). RAW264.7 cells were pretreated with 30 μM of rutin and then exposed to LPS (100 ng/mL) for 12 h. Total cell lysates (30 μg protein) were separated on 10% SDS-PAGE. β-actin was used as the loading control. Proteins were detected by western blotting (upper panels). Protein expression was quantitated by densitometric analysis of the western blots, and is shown in the lower panels (n=3). Significantly different compared with the LPS-stimulated group (p < 0.05 by the paired t-test)

From

Rutin Attenuates Lipopolysaccharide-induced Nitric Oxide Production in Macrophage Cells

Seung-Jae Lee, Seung Yuan Lee, Hyun Joo Ha, Seon Heui Cha, Si Kyung Lee, Sun Jin Hur

Journal of Food and Nutrition Research. 2015, 3(3), 202-205 doi:10.12691/jfnr-3-3-12